The BNIP1 Monoclonal Antibody (CAB4405) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and is validated for use in Western blot applications.BNIP1, also known as Bcl-2/adenovirus E1B 19 kDa protein-interacting protein 1, plays a critical role in regulating cell death and survival pathways. Its interaction with Bcl-2 family members and involvement in mitochondrial dynamics make it a key player in cancer biology and neurodegenerative diseases.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
BNIP1 Monoclonal Antibody
SKU:
CAB4405
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC2137
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. In addition, this protein is involved in vesicle transport into the endoplasmic reticulum. Alternative splicing of this gene results in four protein products with identical N- and C-termini.
Purification Method
Affinity purification
Gene ID
662
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using BNIP1 Rabbit mAb (CAB4405) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using BNIP1 Rabbit mAb (CAB4405) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
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