The Bovine Angiotensin II (AngII) ELISA Kit is a highly reliable and sensitive assay designed for the accurate quantification of Angiotensin II levels in bovine serum, plasma, and cell culture supernatants. This kit offers high specificity and precision, ensuring consistent and reproducible results for a variety of research applications.Angiotensin II is a key peptide hormone involved in regulating blood pressure, fluid balance, and electrolyte homeostasis. It plays a critical role in the renin-angiotensin-aldosterone system, making it a valuable biomarker for studying cardiovascular diseases, hypertension, and kidney disorders.
With this ELISA kit, researchers can accurately measure Angiotensin II levels in bovine samples, providing valuable insights into the physiological and pathological processes influenced by this important hormone. Ideal for both basic research and drug development studies, the Bovine Angiotensin II ELISA Kit is an essential tool for advancing our understanding of Angiotensin II biology and its implications in various health conditions.
Product Name:
Bovine Angiotensin II (AngII) ELISA Kit
Product Code:
BODL00011
Size:
96 Assays
Target:
AngII
Synonyms:
Ang-II, Angiotensin-2
Detection Method:
Competition
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
85-103
94
Heparin plasma (n=5)
87-99
93
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
