Bovine CHI3L1 (Chitinase-3-like protein 1) ELISA Kit
The Bovine CHI3L1 (Chitinase 3-Like Protein 1) ELISA Kit is specially designed for the quantitative detection of CHI3L1 levels in bovine serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications. CHI3L1, also known as YKL-40, is a glycoprotein that plays a crucial role in inflammation, tissue remodeling, and cell proliferation. Elevated levels of CHI3L1 have been linked to various diseases, including cancer, asthma, and inflammatory conditions, making it a valuable biomarker for studying these conditions and assessing disease progression.
By using the Bovine CHI3L1 ELISA Kit, researchers can easily measure CHI3L1 levels in bovine samples, providing valuable insights into disease mechanisms and potential therapeutic targets. This kit is an essential tool for studying the role of CHI3L1 in various physiological and pathological processes in bovine models.
Product Name:
Bovine CHI3L1 (Chitinase-3-like protein 1) ELISA Kit
SKU:
BOFI00053
Reactivity:
Bovine
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.938 ng/mL
Range:
1.563-100 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell Lysis Buffer or Tissue Lysis Buffer, and Other Biological Fluid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
81-94%
89-104%
85-103%
EDTA Plasma (n = 5)
83-92%
86-104%
82-97%
Heparin Plasma (n = 5)
90-97%
94-99%
88-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-100
96
EDTA Plasma (n = 5)
90-105
91
Heparin Plasma (n = 5)
87-103
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
