The Bovine IFNL3 (Interferon Lambda 3) ELISA Kit from Assay Genie is a cutting-edge research tool designed for the precise quantification of IFNL3 levels in bovine samples. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of applications in the field of bovine immunology and virology. IFNL3, also known as Interferon Lambda 3, is a key cytokine involved in the host immune response to viral infections. It plays a crucial role in antiviral defense and modulation of immune responses, making it a valuable marker for studying immune system dynamics in bovine species.
The ability to accurately measure IFNL3 levels using this ELISA kit makes it an indispensable tool for researchers investigating immune responses in bovine livestock, infectious diseases, and vaccine development. With its easy-to-use protocol and reliable performance, the Bovine IFNL3 ELISA Kit is an essential asset for scientists and researchers looking to advance their understanding of bovine immune function and response to viral challenges. Trust Assay Genie's expertise and innovation to drive your research forward with confidence and precision.
Product Name:
Bovine IFNL3 (Interferon lambda 3) ELISA Kit
SKU:
AEFI00013
Reactivity:
Bovine
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
18.75 pg/mL
Range:
31.2-2000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
92-105%
94-101%
91-100%
EDTA Plasma (n = 5)
92-104%
86-100%
85-100%
Heparin Plasma (n = 5)
89-105%
90-100%
86-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
88-105
98
EDTA Plasma (n = 5)
89-102
94
Heparin Plasma (n = 5)
94-105
98
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
