Bovine Retinol Binding Protein 4, Plasma (RBP4) ELISA Kit
The Bovine Retinol-Binding Protein 4 (Plasma RBP4) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of RBP4 levels in bovine plasma samples. This kit is ideal for researchers studying metabolism, obesity, and related conditions as RBP4 is known to be involved in insulin resistance and metabolic disorders.RBP4 is a key protein that regulates retinol transport and metabolism, playing a crucial role in various physiological processes.
Abnormal levels of RBP4 have been linked to insulin resistance, obesity, and other metabolic disorders, making it a valuable biomarker for understanding these conditions and developing targeted therapies.With its reliable performance and ease of use, the Bovine Retinol-Binding Protein 4 (Plasma RBP4) ELISA Kit is a valuable tool for researchers looking to study the role of RBP4 in bovine health and metabolism.
Product Name:
Bovine Retinol Binding Protein 4, Plasma (RBP4) ELISA Kit
Product Code:
BODL00149
Size:
96 Assays
Target:
RBP4
Synonyms:
PRBP, RBP, Plasma retinol-binding protein
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
