Bovine TEK Tyrosine Kinase, Endothelial (Tie2) ELISA Kit
The Bovine TEK (Tyrphostin AG879) Tyrosine Kinase (Endothelial Tie2) ELISA Kit is specifically designed to accurately measure the levels of Tyrosine Kinase (Endothelial Tie2) in bovine serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.Tyrosine Kinase (Endothelial Tie2) is a critical enzyme involved in angiogenesis and vascular development, playing a key role in regulating blood vessel formation and endothelial cell function.
Dysregulation of Tyrosine Kinase (Endothelial Tie2) has been implicated in various diseases and conditions, including cancer, cardiovascular disorders, and neurodegenerative diseases. As such, this ELISA kit serves as a valuable tool for studying these conditions and exploring potential therapeutic interventions.
Product Name:
Bovine TEK Tyrosine Kinase, Endothelial (Tie2) ELISA Kit
Product Code:
BODL00169
Size:
96 Assays
Target:
Tie2
Synonyms:
CD202b, CD202-B, TEK, VMCM1, Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains 2, Venous Malformations Multiple Cutaneous And Mucosal
Detection Method:
Sandwich
Range:
6.25-400ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
87-100
93
Heparin plasma (n=5)
88-100
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
