Bovine Transforming Growth Factor Beta Induced Protein (TGFbI) ELISA Kit
The Bovine Transforming Growth Factor Beta Induced Protein (TGFBI) ELISA Kit is a powerful tool for the precise measurement of TGFBI levels in bovine samples such as serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring the accuracy and consistency of your results for a wide range of research applications.TGFBI is a key protein involved in various biological processes, including cell adhesion, migration, and proliferation. It plays a critical role in tissue development, wound healing, and inflammation, making it a valuable target for studying disease mechanisms and potential therapeutic interventions.
By accurately measuring TGFBI levels, researchers can gain insights into the pathophysiology of conditions such as fibrosis, cancer, and corneal dystrophies, leading to advancements in diagnosis and treatment strategies.Overall, the Bovine Transforming Growth Factor Beta Induced Protein (TGFBI) ELISA Kit is an essential tool for researchers looking to delve deeper into the biology of TGFBI and its potential implications in various disease states.
Product Name:
Bovine Transforming Growth Factor Beta Induced Protein (TGFbI) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
