Bovine Vascular Endothelial Growth Factor D (VEGFD) ELISA Kit
The Bovine Vascular Endothelial Growth Factor D (VEGF-D) ELISA Kit is a powerful tool for the accurate quantification of VEGF-D levels in bovine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reliable results, making it an invaluable asset for various research purposes.VEGF-D is a key factor in the process of angiogenesis, facilitating the growth of blood vessels and impacting cellular proliferation.
Its role in various health conditions like cancer, cardiovascular diseases, and neurodegenerative disorders underscores its importance as a biomarker for understanding these diseases and developing potential interventions.With the Bovine VEGF-D ELISA Kit from AssayGenie, researchers can confidently measure VEGF-D levels in bovine samples, enabling in-depth investigation into the mechanisms of angiogenesis and the development of targeted therapies.
Product Name:
Bovine Vascular Endothelial Growth Factor D (VEGFD) ELISA Kit
Product Code:
BODL00187
Size:
96 Assays
Target:
VEGFD
Synonyms:
FIGF, VEGF-D, C-Fos Induced Growth Factor
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
