Western blot analysis of various lysates, using BRD9 antibody (CAB22755) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The BRD9 Monoclonal Antibody (CAB22755) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced using innovative monoclonal technology, ensuring high purity and specificity for BRD9 detection in various experimental settings.BRD9 is a member of the bromodomain-containing protein family, known for its role in modulating gene expression by recognizing acetylated histones. It is particularly relevant in the context of cancer research, as dysregulation of chromatin remodeling processes can contribute to oncogenesis and tumor progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
BRD9 Monoclonal Antibody
SKU:
CAB22755
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC58081
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PRO9856, SMARCI2, LAVS3040, BRD9
Positive Sample:
Neuro-2a, RAW 264.7, C6, PC-12
Cellular Localization:
Nucleus.
Calculated MW:
67kDa
Observed MW:
80kDa
Enables lysine-acetylated histone binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Located in nucleoplasm. Part of SWI/SNF complex.
Purification Method
Affinity purification
Gene ID
65980
RRID
AB_3662941
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using BRD9 Rabbit mAb (CAB22755) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
