The BRG1/SMARCA4 Monoclonal Antibody (CAB19556) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, boasts high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the BRG1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component of studies in epigenetics, gene expression, and cancer research.BRG1, a member of the SWI/SNF chromatin remodeling complex, plays a crucial role in regulating gene transcription and chromatin structure.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
BRG1/SMARCA4 Monoclonal Antibody
SKU:
CAB19556
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0035
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI,which is required for transcriptional activation of genes normally repressed by chromatin. In addition,this protein can bind BRCA1,as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
6597
RRID
AB_2862665
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat brain using BRG1/SMARCA4 Rabbit mAb (CAB19556) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Western blot analysis of lysates from Mouse brain using BRG1/SMARCA4 Rabbit mAb (CAB19556) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Confocal imaging of NIH-3T3 cells using BRG1/SMARCA4 Rabbit mAb (CAB19556,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
