Anti-BTN2A1 Antibody (CAB10418)

SKU:
CAB10418
€99 - €249

Description

Technical Manual

Anti-BTN2A1 Antibody

Product Name:Anti-BTN2A1 Antibody
Product SKU:CAB10418
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a member of the immunoglobulin superfamily. The gene is located in a cluster of butyrophilin-like genes in the juxta-telomeric region of the major histocompatibility complex on chromosome 6. A pseudogene of this gene has been identified in this cluster. The encoded protein is an integral plasma membrane protein involved in lipid, fatty-acid, and sterol metabolism. Alterations in this gene may be associated with several disease states including metabolic syndrome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-240 of human BTN2A1 (NP_510961.1).
Sequence:

FIVVGPTDPILATVGENTTLRCHLSPEKNAEDMEVRWFRSQFSPAVFVYKGGRERTEEQMEEYRGRTTFVSKDISRGSVALVIHNITAQENGTYRCYFQEGRSYDEAILHLVVAGLGSKPLISMRGHEDGGIRLECISRGWYPKPLTVWRDPYGGVAPALKEVSMPDADGLFMVTTAVIIRDKSVRNMSCSINNTLLGQKKESVIFIPESF

Gene ID:11120
Swiss Prot:Q7KYR7
Synonyms:BTN2A1; BK14H9.1; BT2.1; BTF1; BTN2.1; DJ3E1.1
Calculated MW:37kDa/42kDa/53kDa/59kDa/60kDa
Observed MW:72kDa
Reactivity:Human
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:U-251MG, HT-1080, HT-29, LO2, A-549, 293T
Cellular Location:Membrane, Single-pass type I membrane protein

Product Images

Anti-BTN2A1 Antibody (CAB10418)

Western blot analysis of extracts of various cell lines, using BTN2A1 antibody (CAB10418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Uniprot Information

UniProt Protein Function:BTN2A1: a member of the BTN2 subfamily of genes, which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6, consisting of seven genes belonging to the expanding B7/butyrophilin-like group, a subset of the immunoglobulin gene superfamily. The encoded protein is an integral plasma membrane B box protein involved in lipid, fatty-acid and sterol metabolism. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: integral to plasma membrane

Biological Process: lipid metabolic process

NCBI Summary:This gene encodes a member of the immunoglobulin superfamily. The gene is located in a cluster of butyrophilin-like genes in the juxta-telomeric region of the major histocompatibility complex on chromosome 6. A pseudogene of this gene has been identified in this cluster. The encoded protein is an integral plasma membrane protein involved in lipid, fatty-acid, and sterol metabolism. Alterations in this gene may be associated with several disease states including metabolic syndrome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]
UniProt Code:Q7KYR7
NCBI GenInfo Identifier:215274180
NCBI Gene ID:11120
NCBI Accession:Q7KYR7.3
UniProt Secondary Accession:Q7KYR7,O00475, P78408, Q59EN4, Q7KYQ7, Q7Z386, Q96AV7 Q9NU62, B4DLP9, E9PGR4,
UniProt Related Accession:Q7KYR7
Molecular Weight:527
NCBI Full Name:Butyrophilin subfamily 2 member A1
NCBI Synonym Full Names:butyrophilin, subfamily 2, member A1
NCBI Official Symbol:BTN2A1
NCBI Official Synonym Symbols:BTF1; BT2.1; BTN2.1; DJ3E1.1; BK14H9.1
NCBI Protein Information:butyrophilin subfamily 2 member A1
UniProt Protein Name:Butyrophilin subfamily 2 member A1
Protein Family:Butyrophilin subfamily
UniProt Gene Name:BTN2A1
UniProt Entry Name:BT2A1_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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