Anti-Beta-TRCP Antibody (CAB1656)

Antibody Name:	Anti-Beta-TRCP Antibody
Antibody SKU:	CAB1656
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-340 of human Beta-TRCP (NP_378663.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-340 of human Beta-TRCP (NP_378663.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AFQN SSER EDCN NGEP PRKI IPEK NSLR QTYN SCAR LCLN QETV CLAS TAMK TENC VAKT KLAN GTSS MIVP KQRK LSAS YEKE KELC VKYF EQWS ESDQ VEFV EHLI SQMC HYQH GHIN SYLK PMLQ RDFI TALP ARGL DHIA ENIL SYLD AKSL CAAE LVCK EWYR VTSD GMLW KKLI ERMV RTDS LWRG LAER RGWG QYLF KNKP PDGN APPN SFYR ALYP KIIQ DIET IESN WRCG RHSL QRIH CRSE TSKG VYCL QYDD QKIV SGLR DNTI KIWD KNTL ECKR ILT
Gene ID:	8945
Uniprot:	Q9Y297
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	65kDa/68kDa
Observed MW:	65KDa

Synonyms:

BTRC, BETA-TRCP, FBW1A, FBXW1, FBXW1A, FWD1, bTrCP, bTrCP1, betaTrCP, Beta-TRCP

Background:

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6.

UniProt Protein Function:	BTRC: a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Regulates the stability of CTNNB1 and participates in Wnt signaling. Interacts directly with SKP1 in the SCF complex. Interacts specifically with phosphorylated CTNNB1 and NFKBIA, ubiquitination substrates. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10q24.32 Cellular Component: nucleoplasm; SCF ubiquitin ligase complex; cytosol Molecular Function:protein dimerization activity; protein phosphorylated amino acid binding; protein binding; ubiquitin-protein ligase activity; beta-catenin binding; ligase activity Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; circadian rhythm; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; viral reproduction; regulation of cell cycle; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; protein ubiquitination; positive regulation of circadian rhythm; protein amino acid dephosphorylation; signal transduction; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; G2/M transition of mitotic cell cycle; regulation of I-kappaB kinase/NF-kappaB cascade; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway; protein destabilization; negative regulation of transcription factor activity; mammary gland epithelial cell proliferation; regulation of circadian rhythm; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of smoothened signaling pathway; mitotic cell cycle; negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar 2012]
UniProt Code:	Q9Y297
NCBI GenInfo Identifier:	13124271
NCBI Gene ID:	8945
NCBI Accession:	Q9Y297.1
UniProt Secondary Accession:	Q9Y297,Q5W141, Q5W142, Q9Y213, B5MD49,
UniProt Related Accession:	Q9Y297
Molecular Weight:	605
NCBI Full Name:	F-box/WD repeat-containing protein 1A
NCBI Synonym Full Names:	beta-transducin repeat containing E3 ubiquitin protein ligase
NCBI Official Symbol:	BTRC
NCBI Official Synonym Symbols:	FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP
NCBI Protein Information:	F-box/WD repeat-containing protein 1A; beta-TrCP1; E3RSIkappaB; F-box and WD-repeat protein 1B; epididymis tissue protein Li 2a; pIkappaBalpha-E3 receptor subunit; F-box and WD repeats protein beta-TrCP
UniProt Protein Name:	F-box/WD repeat-containing protein 1A
UniProt Synonym Protein Names:	E3RSIkappaB; Epididymis tissue protein Li 2a; F-box and WD repeats protein beta-TrCP; pIkappaBalpha-E3 receptor subunit
Protein Family:	F-box/WD repeat-containing protein
UniProt Gene Name:	BTRC
UniProt Entry Name:	FBW1A_HUMAN

	Western blot analysis of extracts of U-251MG cells, using Beta-TRCP antibody (CAB1656) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded mouse spleen using Beta-TRCP Rabbit pAb (CAB1656) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using Beta-TRCP Rabbit pAb (CAB1656) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-BTRC Antibody (CAB18232)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)