Anti-CALB1 Antibody (CAB0802)

Antibody Name:	Anti-CALB1 Antibody
Antibody SKU:	CAB0802
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human CALB1 (NP_004920.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, Mouse eye, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human CALB1 (NP_004920.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAES HLQS SLIT ASQF FEIW LHFD ADGS GYLE GKEL QNLI QELQ QARK KAGL ELSP EMKT FVDQ YGQR DDGK IGIV ELAH VLPT EENF LLLF RCQQ LKSC EEFM KTWR KYDT DHSG FIET EELK NFLK DLLE KANK TVDD TKLA EYTD LMLK LFDS NNDG KLEL TEMA RLLP VQEN FLLK FQGI KMCG KEFN KAFE LYDQ DGNG YIDE NELD ALLK DLCE KNKQ DLDI NNIT TYKK NIMA LSDG GKLY RTDL ALIL CAGD N
Gene ID:	793
Uniprot:	P05937
Cellular Location:
Calculated MW:	23kDa/30kDa
Observed MW:	30kDa

Synonyms:

CALB1, CALB, D-28K, calbindin, Calbindin

Background:

The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease.

UniProt Protein Function:	CALB1: Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Interacts with RANBP9. Belongs to the calbindin family.
UniProt Protein Details:	Protein type:Calcium-binding Chromosomal Location of Human Ortholog: 8q21.3 Cellular Component: cell soma; axon; dendrite; synapse; nucleus; cytosol Molecular Function:vitamin D binding; protein binding; zinc ion binding; calcium ion binding Biological Process: cytosolic calcium ion homeostasis; long-term memory; short-term memory; regulation of synaptic plasticity; locomotory behavior
NCBI Summary:	Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. [provided by RefSeq, Jul 2008]
UniProt Code:	P05937
NCBI GenInfo Identifier:	115394
NCBI Gene ID:	793
NCBI Accession:	P05937.2
UniProt Secondary Accession:	P05937,B2R696, B7Z9J4,
UniProt Related Accession:	P05937
Molecular Weight:	23,609 Da
NCBI Full Name:	Calbindin
NCBI Synonym Full Names:	calbindin 1, 28kDa
NCBI Official Symbol:	CALB1
NCBI Official Synonym Symbols:	CALB
NCBI Protein Information:	calbindin; D-28K; calbindin D28; RTVL-H protein; calbindin 1, (28kD); vitamin D-dependent calcium-binding protein, avian-type
UniProt Protein Name:	Calbindin
UniProt Synonym Protein Names:	Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type
Protein Family:	Calbindin
UniProt Gene Name:	CALB1
UniProt Entry Name:	CALB1_HUMAN

	Western blot analysis of extracts of various cell lines, using CALB1 antibody (CAB0802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat kidney using CALB1 antibody (CAB0802) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using CALB1 antibody (CAB0802) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-CALB1 Antibody (CAB15035)	Human Calbindin / CALB1 ELISA Kit
Anti-Calbindin Antibody (CAB4284)	Human Calbindin (CALB1) ELISA Kit
CALB1 Antibody (PACO01922)
CALB1 Antibody (PACO08142)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)