CaMK1-alpha Colorimetric Cell-Based ELISA Kit

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SKU:
CBCAB00558
€499

Description

Technical Manual

CaMK1-alpha Cell-Based ELISA Kit

The CaMK1-alpha Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CaMK1-alpha protein expression profile in cells. The kit can be used for measuring the relative amounts of CaMK1-alpha in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CaMK1-alpha .

How does our CaMK1-alpha Colorimetric Cell-Based ELISA Kit work?

Qualitative determination of CaMK1-alpha concentration is achieved by an indirect ELISA format. In essence, CaMK1-alpha is captured by CaMK1-alpha -specific primary (1°) antibodies while the HRP-conjugated secondary (2°) antibodies bind the Fc region of the 1° antibody. Through this binding, the HRP enzyme conjugated to the 2° antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

CaMK1-alpha Colorimetric Cell-Based ELISA Kit - Information

Product Name:CaMK1-alpha Colorimetric Cell-Based ELISA Kit
Product Code:/SKUCBCAB00558
Description:The CaMK1-alpha Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect CaMK1-alpha protein expression profile in cells. The kit can be used for measuring the relative amounts of CaMK1-alpha in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on CaMK1-alpha.
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nm
Storage/Stability:4°C/6 Months
Reactivity:Human, Mouse, Rat
Assay Type:Cell-Based
Database Links:Gene ID: 8536, UniProt ID: Q14012, OMIM #: 604998, Unigene #: Hs.434875
Format:96-Well Microplate
NCBI Gene Symbol:CAMK1
Sub Type:None
Target Name:CaMK1-alpha

Kit Principle

Figure: Schematic representation of ELISA Genie Cell-Based Colorimetric ELISA principle

Kit components

Quantity

96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 ml
Quenching Buffer24 ml
Blocking Buffer50 ml
15X Wash Buffer50 ml
Primary Antibody Diluent12 ml
100x Anti-Phospho Target Antibody 60 ul
100x Anti-Target Antibody60 ul
Anti-GAPDH Antibody60 ul
HRP-Conjugated Anti-Rabbit IgG Antibody12 ml
HRP-Conjugated Anti-Mouse IgG Antibody12 ml
SDS Solution12 ml
Stop Solution24 ml
Ready-to-Use Substrate12 ml
Crystal Violet Solution12 ml
Adhesive Plate Seals2 seals

Additional equipment and materials required

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µl to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

Kit Protocol

This is a summarized version of the kit protocol. Please view the technical manual of this kit for information on sample preparation, reagent preparation and plate lay out.

1.Seed 200 µl of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µl of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µl of 1x TBS, twice.
5.Fix the cells by incubating with 100 µl of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µl 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week.
7.Add 100 µl of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µl of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µl of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µl of 1x primary antibodies (Anti-CaMK1-alpha Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µl of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µl of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µl of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µl of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µl of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

CaMK1-alpha - Protein Information

UniProt Protein Function:CAMK1A: an ubiquitous protein kinase of the CAMK1 family. Activated by Ca(2+)/calmodulin. Must be phosphorylated to be maximally active. Substrates include synapsin I.
UniProt Protein Details:

Protein type:Kinase, protein; EC 2.7.11.17; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; CAMK group; CAMK1 family

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cytoplasm; nucleus

Molecular Function:calmodulin binding; protein binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: positive regulation of syncytium formation by plasma membrane fusion; regulation of muscle cell differentiation; nucleocytoplasmic transport; signal transduction; cell cycle; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; positive regulation of synapse structural plasticity; positive regulation of protein export from nucleus; regulation of protein localization; regulation of protein binding; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein binding

NCBI Summary:Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008]
UniProt Code:Q14012
NCBI GenInfo Identifier:3122301
NCBI Gene ID:8536
NCBI Accession:Q14012.1
UniProt Secondary Accession:Q14012,Q3KPF6,
UniProt Related Accession:Q14012
Molecular Weight:370
NCBI Full Name:Calcium/calmodulin-dependent protein kinase type 1
NCBI Synonym Full Names:calcium/calmodulin-dependent protein kinase I
NCBI Official Symbol:CAMK1  
NCBI Official Synonym Symbols:CAMKI  
NCBI Protein Information:calcium/calmodulin-dependent protein kinase type 1; caM-KI; caMKI-alpha; caM kinase I alpha
UniProt Protein Name:Calcium/calmodulin-dependent protein kinase type 1
UniProt Synonym Protein Names:CaM kinase I; CaM-KI; CaM kinase I alpha; CaMKI-alpha
Protein Family:Calcium/calmodulin-dependent serine/threonine-protein kinase
UniProt Gene Name:CAMK1  
UniProt Entry Name:KCC1A_HUMAN
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