The CAMP (Cathelicidin Antimicrobial Peptide) ELISA Kit is a valuable tool for researchers looking to accurately measure levels of CAMP in various biological samples. This kit offers exceptional sensitivity and specificity, allowing for precise and reliable results for a variety of applications.CAMP is an important component of the innate immune system, playing a key role in defense against microbial pathogens. Its levels have been linked to various inflammatory and infectious diseases, making it a valuable biomarker for studying immune responses and developing new therapeutic strategies. With easy-to-follow instructions and quick assay times, the CAMP ELISA Kit from Assay Genie is an indispensable resource for researchers working in immunology, infectious diseases, and antimicrobial research. Order yours today and enhance your research efforts with accurate measurements of CAMP levels.
Product Name:
cAMP (Cyclic adenosine monophosphate) ELISA Kit
SKU:
UNES00036
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.