Canakinumab ELISA Kit (Ilaris®) Qualitative

SKU:
HUMB00057
Product type:
Biosimilar ELISA
Application:
ELISA
Reactivity:
Human
€899

Description

Canakinumab (Ilaris®) Antibody screening - Qualitative ELISA Kit

Assay Genie Qualitative Antibodies to Canakinumab ELISA has been especially developed for the qualitative analysis of antibodies to canakinumab in serum and plasma samples. Assay Genie Qualitative Antibodies to Canakinumab ELISA is optimized with Ilaris®

Canakinumab (Ilaris®) Antibody screening - Qualitative ELISA Kit test principle

Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Controls and samples (serum or plasma) are incubated in the microtiter plate coated with the drug canakinumab. After incubation, the wells are washed. Then, horse radish peroxidase (HRP) conjugated probe is added and binds to canakinumab antibodies captured by the drug canakinumab on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. Finally, the reaction is terminated with an acidic stop solution. The colour developed is proportional to the amount of canakinumab antibodies in the sample or controls. The results can be evaluated with using cutoff value.

Canakinumab (Ilaris®) Antibody screening - Qualitative ELISA Kit Product Infromation

Key Information Description

Application

Antibody screening - Qualitative

Required Volume

10 µl

Total Time

140 minutes

Sample Type

Serum, Plasma

Number of Assays

96

Detection Limit

+ / - ng/mL

Spike Recovery

-

Shelf Life (year)

1

Alternative Names

-

About Canakinumab (Ilaris®) Antibody screening - Qualitative ELISA Kit

Canakinumab is a recombinant, human anti-human-IL-1β monoclonal antibody that belongs to the IgG1/κ isotype subclass. Canakinumab binds to human IL-1β and neutralizes its inflammatory activity by blocking its interaction with IL-1 receptors, but it does not bind IL-1alpha or IL-1 receptor antagonist (IL-1ra).

In inflammatory diseases involving Cryopyrin-Associated Periodic Syndromes (CAPS), interleukin-1 beta (IL-1β) is excessively activated and drives inflammation. The protein cryopyrin controls the activation of IL-1β, and mutations in cryopyrin's gene, NLRP-3, up-regulate IL-1β activation. Canakinumab is a human monoclonal anti-human IL-1β antibody of the IgG1/κ isotype. Canakinumab binds to human IL-1β and neutralizes its inflammatory activity by blocking its interaction with IL-1 receptors, but it does not bind IL-1α or IL-1 receptor antagonist (IL-1ra).

Assay Genie ELISA kits can be used for drug level and anti-drug antibodies measurements.

Assay Genie Canakinumab ELISA products:

Product Application SKU

Canakinumab (Ilaris®)

Free Drug

Canakinumab (Ilaris®)

Antibody screening - Qualitative

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