The Canine Cholecystokinin (CCK) ELISA Kit is specifically designed to accurately measure levels of cholecystokinin in canine serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Cholecystokinin is a vital peptide hormone that plays a key role in digestion and the regulation of appetite. It is involved in signaling the release of digestive enzymes and bile from the pancreas and gallbladder, as well as the feeling of satiety. Understanding the levels of cholecystokinin in canine samples can provide valuable insights into gastrointestinal health and metabolic disorders in dogs.
Whether studying digestive disorders, metabolic diseases, or the effects of diet and nutrition on canine health, the Canine Cholecystokinin ELISA Kit is a valuable tool for researchers and veterinarians alike. With its accuracy and reliability, this kit is essential for advancing our understanding of canine physiology and developing effective treatment strategies for various canine health conditions.
Product Name:
Canine Cholecystokinin (CCK) ELISA Kit
Product Code:
CNDL00016
Size:
96 Assays
Target:
CCK
Synonyms:
CCK-PZ
Detection Method:
Competition
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
