The Canine Glucose Transporter 4 (GLUT4) ELISA Kit is a powerful tool for accurately measuring levels of GLUT4 in canine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees precise and consistent results, making it an invaluable asset for various research applications.GLUT4 is a key player in glucose metabolism, facilitating the uptake of glucose into cells and regulating blood sugar levels.
Dysregulation of GLUT4 has been implicated in conditions such as diabetes and obesity, highlighting the importance of studying GLUT4 levels in canine models for better understanding and treatment of these diseases.Whether investigating canine metabolic disorders or exploring potential therapeutic interventions, the Canine GLUT4 ELISA Kit provides researchers with a reliable and efficient tool for conducting their studies with confidence.
Product Name:
Canine Glucose Transporter 4 (GLUT4) ELISA Kit
Product Code:
CNDL00030
Size:
96 Assays
Target:
GLUT4
Synonyms:
SLC2A4, Solute carrier family 2, facilitated glucose transporter member 4, Glucose transporter type 4, insulin-responsive
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
