Canine High Mobility Group AT Hook Protein 1 (HMGA1) ELISA Kit
The Canine High Mobility Group AT-hook Protein 1 (HMGA1) ELISA Kit is specifically designed for the accurate measurement of HMGA1 levels in canine serum, plasma, and tissue lysates. With its high sensitivity and specificity, this kit ensures precise and reliable results, making it a valuable tool for a variety of research purposes.HMGA1 is a multifunctional protein that plays a crucial role in gene regulation, cell differentiation, and tumorigenesis. It is known to be involved in various biological processes, making it a key target for studying developmental disorders, cancer, and other diseases.
By accurately quantifying HMGA1 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and explore potential therapeutic interventions.Overall, the Canine HMGA1 ELISA Kit offers researchers a powerful tool for studying HMGA1 biology in canine models, advancing our understanding of disease mechanisms, and potentially identifying new therapeutic targets. Order now and take your research to the next level with this cutting-edge ELISA kit from Assay Genie.
Product Name:
Canine High Mobility Group AT Hook Protein 1 (HMGA1) ELISA Kit
Product Code:
CNDL00035
Size:
96 Assays
Target:
HMGA1
Synonyms:
HMG-R, HMGIY, High mobility group protein A1, High mobility group protein R, High mobility group protein HMG-I/HMG-Y
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
