The Canine Interleukin-1 Beta (IL1B) ELISA Kit is a reliable and accurate tool for the measurement of IL1B levels in canine serum, plasma, and tissue culture supernatants. With high sensitivity and specificity, this kit provides consistent and reproducible results, making it ideal for various research applications.IL1B is a key cytokine involved in the regulation of inflammatory responses and immune system function in dogs. Its dysregulation has been implicated in various canine diseases, including arthritis, dermatitis, and autoimmune disorders.
Understanding IL1B levels in canine samples can provide valuable insights into the pathogenesis of these conditions and facilitate the development of targeted therapies.Overall, the Canine Interleukin-1 Beta (IL1B) ELISA Kit is a valuable tool for researchers studying canine immunology and inflammatory diseases, offering a reliable method for quantifying IL1B levels in biological samples.
Product Name:
Canine Interleukin 1 Beta (IL-1b) ELISA Kit for
Product Code:
CNDL00048
Size:
96 Assays
Target:
IL1b
Synonyms:
IL1-B, IL1-Beta, IL1F2, IL-1?, Interleukin-1 Family Member 2, Catabolin
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
