Canine MIP-1beta (Macrophage Inflammatory Protein 1 Beta) ELISA Kit
The Canine MIP-1 (Macrophage Inflammatory Protein-1) Beta ELISA Kit is a cutting-edge tool specifically designed for the precise measurement of MIP-1 levels in canine samples such as serum, plasma, and cell culture supernatants. This advanced kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a diverse array of research endeavors. Macrophage Inflammatory Protein-1 Beta (MIP-1β) is a critical chemokine involved in inflammatory responses, particularly in recruiting and activating immune cells like macrophages and lymphocytes. Its role in immune regulation and inflammatory processes makes it a pivotal player in various canine health conditions, including infectious diseases, autoimmune disorders, and inflammatory conditions.
With its ability to detect and quantify canine MIP-1β levels with high precision, this ELISA kit is an invaluable asset for researchers and clinicians looking to unravel the intricate mechanisms of canine immune responses and inflammation. By utilizing this kit, researchers can gain deeper insights into the role of MIP-1β in canine health and disease, paving the way for novel therapeutic interventions and tailored treatment strategies.
Product Name:
Canine MIP-1beta (Macrophage Inflammatory Protein 1 Beta) ELISA Kit
SKU:
AEFI00043
Reactivity:
Canine
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
4.688 pg/mL
Range:
7.813-500 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-99%
86-95%
88-94%
EDTA Plasma (n = 5)
88-103%
83-99%
86-94%
Heparin Plasma (n = 5)
89-100%
83-101%
84-97%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
88-104
96
EDTA Plasma (n = 5)
90-99
97
Heparin Plasma (n = 5)
87-104
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
