SynonymsCCL2,GDCF2,HC11,HSMCR30,MCAF,MCP1,SCYA2,SMC-CF,Chemokine C-C-Motif Ligand 2,Monocyte Chemotactic And Activating Factor,Monocyte Secretory Protein JE
ReactivityCanine
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
SKU:
CNDL00062
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P52203
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CCL2,GDCF2,HC11,HSMCR30,MCAF,MCP1,SCYA2,SMC-CF,Chemokine C-C-Motif Ligand 2,Monocyte Chemotactic And Activating Factor,Monocyte Secretory Protein JE
Reactivity:
Canine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
Canine Monocyte Chemotactic Protein 1 (MCP1) ELISA Kit
The Canine Monocyte Chemotactic Protein-1 (MCP1) ELISA kit, available at assaygenie.com, is designed for the accurate detection of MCP1 levels in canine serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.MCP1 is an important protein involved in the recruitment and activation of monocytes, playing a crucial role in inflammatory responses and immune regulation in canines.
Elevated levels of MCP1 have been linked to various diseases and conditions, making it a valuable biomarker for studying inflammatory processes and potential therapeutic interventions in canine health.Overall, the Canine MCP1 ELISA kit from AssayGenie provides researchers with a powerful tool for studying MCP1 levels in canines, offering valuable insights into inflammatory responses and immune regulation in our furry companions.
Product Name:
Canine Monocyte Chemotactic Protein 1 (MCP1) ELISA Kit
Product Code:
CNDL00062
Size:
96 Assays
Target:
MCP1-Ca
Synonyms:
CCL2, GDCF2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF, Chemokine C-C-Motif Ligand 2, Monocyte Chemotactic And Activating Factor, Monocyte Secretory Protein JE
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
