Anti-CAPN2 Antibody (CAB1861)

Antibody Name:	Anti-CAPN2 Antibody [KO Validated]
Antibody SKU:	CAB1861
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 350-680 of human CAPN2 (NP_001739.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	H460, A-549, HeLa, Mouse kidney, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 350-680 of human CAPN2 (NP_001739.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SDTY KKWK LTKM DGNW RRGS TAGG CRNY PNTF WMNP QYLI KLEE EDED EEDG ESGC TFLV GLIQ KHRR RQRK MGED MHTI GFGI YEVP EELS GQTN IHLS KNFF LTNR ARER SDTF INLR EVLN RFKL PPGE YILV PSTF EPNK DGDF CIRV FSEK KADY QAVD DEIE ANLE EFDI SEDD IDDG FRRL FAQL AGED AEIS AFEL QTIL RRVL AKRQ DIKS DGFS IETC KIMV DMLD SDGS GKLG LKEF YILW TKIQ KYQK IYRE IDVD RSGT MNSY EMRK ALEE AGFK MPCQ LHQV IVAR FADD QLII DFDN FVRC LVRL ETLF KIFK QLD
Gene ID:	824
Uniprot:	P17655
Cellular Location:	Cell membrane, Cytoplasm
Calculated MW:	71kDa/79kDa
Observed MW:	80kDa

Synonyms:

CAPN2, CANP2, CANPL2, CANPml, mCANP, calpain 2

Background:

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.
NCBI Summary:	The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Code:	P17655
NCBI GenInfo Identifier:	317373596
NCBI Gene ID:	824
NCBI Accession:	P17655.6
UniProt Secondary Accession:	P17655,Q16738, Q6PJT3, Q8WU26, Q9HBB1, A6NDG7, B7ZA96 E7ES58,
UniProt Related Accession:	P17655
Molecular Weight:	71,476 Da
NCBI Full Name:	Calpain-2 catalytic subunit
NCBI Synonym Full Names:	calpain 2
NCBI Official Symbol:	CAPN2
NCBI Official Synonym Symbols:	CANP2; mCANP; CANPL2; CANPml
NCBI Protein Information:	calpain-2 catalytic subunit
UniProt Protein Name:	Calpain-2 catalytic subunit
UniProt Synonym Protein Names:	Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain; M-calpain
Protein Family:	Calpain
UniProt Gene Name:	CAPN2

	Western blot analysis of extracts of various cell lines, using CAPN2 antibody (CAB1861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts from normal (control) and CAPN2 knockout (KO) 293T cells, using CAPN2 antibody (CAB1861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of U2OS cells using CAPN2 antibody (CAB1861) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-Calpain 2 Antibody (CAB4066)	Human CAPN2 / Calpain 2 ELISA Kit
CAPN2 Antibody (PACO05436)	Human Calpain-2 catalytic subunit (CAPN2) ELISA Kit
CAPN2 Antibody (PACO08171)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)