Anti-CAPZA2 Antibody (CAB2054)

Antibody Name:	Anti-CAPZA2 Antibody
Antibody SKU:	CAB2054
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human CAPZA2 (NP_006127.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, THP-1, 22Rv1, Mouse brain, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human CAPZA2 (NP_006127.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADL EEQL SDEE KVRI AAKF IIHA PPGE FNEV FNDV RLLL NNDN LLRE GAAH AFAQ YNLD QFTP VKIE GYED QVLI TEHG DLGN GKFL DPKN RICF KFDH LRKE ATDP RPCE VENA VESW RTSV ETAL RAYV KEHY PNGV CTVY GKKI DGQQ TIIA CIES HQFQ AKNF WNGR WRSE WKFT ITPS TTQV VGIL KIQV HYYE DGNV QLVS HKDI QDSL TVSN EVQT AKEF IKIV EAAE NEYQ TAIS ENYQ TMSD TTFK ALRR QLPV TRTK IDWN KILS YKIG KEMQ NA
Gene ID:	830
Uniprot:	P47755
Cellular Location:
Calculated MW:	19kDa/32kDa
Observed MW:	38kDa

Synonyms:	CAPZA2, CAPPA2, CAPZ
Background:	The protein encoded by this gene is a member of the F-actin capping protein alpha subunit family. It is the alpha subunit of the barbed-end actin binding protein Cap Z. By capping the barbed end of actin filaments, Cap Z regulates the growth of the actin filaments at the barbed end.

UniProt Protein Function:	CAPZA2: F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Belongs to the F-actin-capping protein alpha subunit family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 7q31.2-q31.3 Cellular Component: cortical cytoskeleton; F-actin capping protein complex; membrane; extracellular region; cytosol; actin cytoskeleton Molecular Function:actin binding Biological Process: innate immune response; protein complex assembly; cell motility; barbed-end actin filament capping; blood coagulation
NCBI Summary:	The protein encoded by this gene is a member of the F-actin capping protein alpha subunit family. It is the alpha subunit of the barbed-end actin binding protein Cap Z. By capping the barbed end of actin filaments, Cap Z regulates the growth of the actin filaments at the barbed end. [provided by RefSeq, Jul 2008]
UniProt Code:	P47755
NCBI GenInfo Identifier:	1345695
NCBI Gene ID:	830
NCBI Accession:	P47755.3
UniProt Secondary Accession:	P47755,B4DG50,
UniProt Related Accession:	P47755
Molecular Weight:	286
NCBI Full Name:	F-actin-capping protein subunit alpha-2
NCBI Synonym Full Names:	capping protein (actin filament) muscle Z-line, alpha 2
NCBI Official Symbol:	CAPZA2
NCBI Official Synonym Symbols:	CAPZ; CAPPA2
NCBI Protein Information:	F-actin-capping protein subunit alpha-2; capZ alpha-2; F-actin capping protein alpha-2 subunit
UniProt Protein Name:	F-actin-capping protein subunit alpha-2
UniProt Synonym Protein Names:	CapZ alpha-2
Protein Family:	F-actin-capping protein
UniProt Gene Name:	CAPZA2
UniProt Entry Name:	CAZA2_HUMAN

	Western blot analysis of extracts of various cell lines, using CAPZA2 antibody (CAB2054) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded Human colon using CAPZA2 Rabbit pAb (CAB2054) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse kidney using CAPZA2 Rabbit pAb (CAB2054) at dilution of 1:50 (40x lens).

Antibodies

CAPZA2 Antibody (PACO08183)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)