Description
cas13a Recombinant Protein (N-His)
cas13a Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Val17-Asn319, with an N-terminal His tag. Predicted molecular weight: 37.41 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. CRISPR-associated endoribonuclease Cas13a is a ~168 kDa protein. CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). For research use only.
CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. Unlike many single-component effectors, this CRISPR-Cas system targets RNA. CRISPR clusters are transcribed from pre-CRISPR RNA (crRNA) and processed into crRNA by this protein. Cleaves linear target ssRNA in a pre-crRNA-dependent fashion, preferentially around A residues.
| Product Name: | cas13a Recombinant Protein (N-His) |
| SKU: | GXRP09962 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Lachnospiraceae bacterium |
| Synonyms: | CRISPR-associated endoribonuclease Cas13a; EndoRNase; 3.1.-.-; LbaCas13a; cas13a |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Val17-Asn319 |
| Calculated MW: | 37.41 kDa |
| UniProt: | P0DPB7 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
