The CAS9 Antibody (CAB14997) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to Cas9 protein, enabling researchers to detect and analyze Cas9 expression in various cell types. Validated for use in Western blot applications, the Cas9 Polyclonal Antibody is essential for studies in molecular biology, genetics, and biotechnology.Cas9 is an RNA-guided endonuclease that plays a crucial role in the CRISPR/Cas9 system by creating double-strand breaks in DNA, allowing for precise modification of genes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Other (Wide Range Predicted) samples.
Product Name:
CAS9 Antibody
SKU:
CAB14997
Size:
20μL, 100μL
Reactivity:
Human, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CAS9
Positive Sample:
293T
Observed MW:
160kDa
Staphylococcus aureus Cas9 (SaCas9) is an RNA-guided endonuclease that targets complementary DNA adjacent to a protospacer adjacent motif (PAM) for cleavage. Its small size facilitates in vivo delivery for genome editing in various organisms.
Purification Method
Affinity purification
Gene ID
901176
RRID
AB_2761880
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of extracts of 293T cells and 293T transfected with CAS9 protein, using CAS9 Rabbit pAb (CAB14997) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 120s.
