The Caspase-6 Monoclonal Antibody (CAB19552) is a high-quality antibody developed for reliable detection and analysis of target proteins. Caspase-6 is a key player in the cascade of events leading to programmed cell death, making this antibody essential for investigating these pathways in various cell types.This antibody, developed using rabbits, offers high specificity and sensitivity for detecting Caspase-6 protein in human samples. Validated for Western blot applications, it allows for precise detection and analysis of Caspase-6 levels in experimental settings. Caspase-6 is particularly relevant in cancer research, where dysregulation of apoptosis pathways can contribute to tumorigenesis and resistance to therapy.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Caspase-6 Monoclonal Antibody
SKU:
CAB19552
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC0031
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MCH2, CSP-6, caspase-6, Caspase-6
Positive Sample:
Jurkat, A549, MCF7
Cellular Localization:
Cytoplasm.
Calculated MW:
33kDa
Observed MW:
33kDa
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms.
Purification Method
Affinity purification
Gene ID
839
RRID
AB_2862664
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Caspase-6 Rabbit mAb (CAB19552) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
Immunofluorescence analysis of C6 cells using Caspase-6 Rabbit pAb (CAB19552) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
