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CAST Rabbit Polyclonal Antibody (CAB0097)

SKU:
CAB0097
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 1 Anti-CAST Antibody CAB0097
  • Cell Biology Antibodies 1 Anti-CAST Antibody CAB0097
  • Cell Biology Antibodies 1 Anti-CAST Antibody CAB0097
  • Cell Biology Antibodies 1 Anti-CAST Antibody CAB0097
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:CAST Rabbit Polyclonal Antibody
SKU:CAB0097
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 596-695 of human CAST (NP_001177371.1).
Sequence:ERDD TIPP EYRH LLDD NGQD KPVK PPTK KSED SKKP ADDQ DPID ALSG DLDS CPST TETS QNTA KDKC KKAA SSSK APKN GGKA KDSA KTTE ETSK PKDD
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:200 - 1:2000 IHC-P,1:50 - 1:200
Synonyms:BS-17; PLACK; CAST
Positive Sample:NCI-H460
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol, endoplasmic reticulum
Calculated MW:77kDa
Observed MW:124kDa

The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.

Purification Method:Affinity purification
Gene ID:831
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from H460 cells, using CAST Rabbit pAb (CAB0097) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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