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CAST Rabbit Polyclonal Antibody (CAB16789)

SKU:
CAB16789
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:CAST Rabbit Polyclonal Antibody
SKU:CAB16789
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human CAST (NP_001177371.1).
Sequence:MNPT ETKA IPVS QQME GPHL PNKK KHKK QAVK TEPE KKSQ STKL SVVH EKKS QEGK PKEH TEPK SLPK QASD TGSN DAHN KKAV SRSA EQQP SEKS TEPK TKPQ DMIS AGGE SVAG ITAI SGKP GDKK KEKK SLTP AVPV ESKP DKPS GKSG MDAA LDDL IDTL GGPE ETEE ENTT YTGP EVSD PMSS TYIE ELGK REVT IPPK YREL LAKP IGPD DAID ALSS DFTC GSPT AAGK KTEK EEST EVLK AQSA GTVR SAAP PQEK KRKV EKDT MSDQ ALEA LSAS LGTR QAEP ELDL RSIK EVDE AKAK EEKL EKCG EDDE TIPS EYRL KPAT DKDG KPLL PEPE EKPK PR
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:BS-17; PLACK; CAST
Positive Sample:HeLa,NCI-H460
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol, endoplasmic reticulum
Calculated MW:77kDa
Observed MW:120kDa

The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.

Purification Method:Affinity purification
Gene ID:831
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using CAST Rabbit pAb (CAB16789) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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