Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.3 Monoclonal Antibody, Clone S48 (STMA00081). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.3 Monoclonal Antibody (STMA00081) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT.
Cav1.3 Antibody [S48] is a mouse recombinant monoclonal antibody directed against Cav1.3, offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF, IP and AM. Reported reactivity: Human, Mouse and Rat. Supplied as IgG2a Kappa, purified by Protein G.
Cav1.3, encoded by the CACNA1D gene, is a voltage-gated L-type calcium channel subunit predominantly expressed in neurons and neuroendocrine cells. It plays a vital role in regulating calcium influx, which is essential for neuronal excitability, synaptic plasticity, gene expression, and neurosecretion. In the central nervous system, Cav1.3 contributes to pacemaker activity in dopaminergic neurons and modulates long-term potentiation—key processes underlying learning and memory. Dysregulation of Cav1.3-mediated calcium signaling has been implicated in several neurodegenerative and neuropsychiatric disorders, including Parkinson’s disease, Alzheimer’s disease, and autism spectrum disorders. Its role in dopaminergic neuron vulnerability makes it a potential therapeutic target in Parkinsonian syndromes. Beyond the brain, Cav1.3 is also expressed in the cochlea, where it is essential for auditory transduction in inner hair cells and electromotility in outer hair cells—highlighting its importance in sensory processing. Additionally, its presence in cardiac atrial tissue suggests a role in atrial excitability and arrhythmogenesis, particularly in atrial fibrillation. Given its broad physiological roles and emerging links to neurological disease, Cav1.3 is a critical focus in neuroscience and neurodegeneration research. Targeting Cav1.3 may offer novel strategies for modulating calcium-dependent signaling pathways in both central and peripheral disorders. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
