Description
CBLB/RNF56 Recombinant Protein (N-His)
CBLB/RNF56 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Arg21-Ser433, with an N-terminal His tag. Predicted molecular weight: 49.95 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. E3 ubiquitin-protein ligase CBL-B is a ~109 kDa protein. E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. For research use only.
Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy.
| Product Name: | CBLB/RNF56 Recombinant Protein (N-His) |
| SKU: | GXRP09952 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Mouse |
| Synonyms: | E3 ubiquitin-protein ligase CBL-B, EC:2.3.2.27, Casitas B-lineage lymphoma proto-oncogene b, RING finger protein 56, RING-type E3 ubiquitin transferase CBL-B, SH3-binding protein CBL-B, Signal transduction protein CBL-B, CBLB, RNF56 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Arg21-Ser433 |
| Calculated MW: | 49.95 kDa |
| UniProt: | Q13191 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
