The CBS Antibody (CAB1427) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and is suitable for use in Western blot applications. By binding to the CBS protein, this antibody allows for the detection and analysis of CBS levels in various cell types, making it ideal for research in fields such as metabolism, biochemistry, and genetic disorders.CBS, also known as cystathionine beta-synthase, plays a pivotal role in maintaining the balance of sulfur-containing amino acids in the body.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CBS Antibody
SKU:
CAB1427
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CBSL, HIP4, CBS
Positive Sample:
HepG2, 293T, HeLa, MCF7, Mouse liver, Rat liver, Rat kidney
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
61kDa
Observed MW:
61kDa
The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. This gene is a major contributor to cellular hydrogen sulfide production. Multiple alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
875
RRID
AB_2761132
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CBS Rabbit pAb (CAB1427) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of paraffin-embedded rat liver using CBS Rabbit pAb (CAB1427) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
