The CD11a/LFA-1A/ITGAL Monoclonal Antibody (CAB19009) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in a variety of applications, including Western blotting.CD11a, also known as integrin alpha L, plays a key role in immune cell interactions and migration, making it a crucial target for studies in immunology and inflammatory diseases. By binding to CD11a, this antibody allows for the detection and analysis of this protein in various cell types, providing valuable insights into immune cell function and dysfunction.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD11a/LFA-1A/ITGAL Monoclonal Antibody
SKU:
CAB19009
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0344
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD11A, LFA-1, LFA1A, CD11a/LFA-1A/ITGAL
Positive Sample:
THP-1
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
129kDa
Observed MW:
180kDa
ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
3683
RRID
AB_2862501
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from THP-1 cells, using CD11a/LFA-1A/ITGAL Rabbit mAb (CAB19009) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Human spleen using CD11a/LFA-1A/ITGAL Rabbit mAb (CAB19009) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
