Anti-CD1D Antibody (CAB1760)

Antibody Name:	Anti-CD1D Antibody
Antibody SKU:	CAB1760
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-190 of human CD1D (NP_001757.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	K-562, Jurkat, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-190 of human CD1D (NP_001757.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EVPQ RLFP LRCL QISS FANS SWTR TDGL AWLG ELQT HSWS NDSD TVRS LKPW SQGT FSDQ QWET LQHI FRVY RSSF TRDV KEFA KMLR LSYP LELQ VSAG CEVH PGNA SNNF FHVA FQGK DILS FQGT SWEP TQEA PLWV NLAI QVLN QDKW TRET VQWL LNGT CPQF VSG
Gene ID:	912
Uniprot:	P15813
Cellular Location:	Cell membrane, Endosome membrane, Lysosome membrane, Single-pass type I membrane protein
Calculated MW:	37kDa
Observed MW:	36kDa

Synonyms:

CD1D, CD1A, R3, R3G1

Background:

This gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CD1D: Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
UniProt Protein Details:	Protein type:Apoptosis; Membrane protein, integral; Lipid-binding; Cell surface Chromosomal Location of Human Ortholog: 1q23.1 Cellular Component: cell surface; lysosomal membrane; integral to plasma membrane; cytoplasm; endosome membrane Molecular Function:histone binding; beta-2-microglobulin binding; exogenous lipid antigen binding; lipid antigen binding; cell adhesion molecule binding; receptor activity; endogenous lipid antigen binding Biological Process: antigen processing and presentation, endogenous lipid antigen via MHC class Ib; heterotypic cell-cell adhesion; detection of bacterium; viral reproduction; positive regulation of innate immune response; antigen processing and presentation, exogenous lipid antigen via MHC class Ib; T cell selection; innate immune response; positive regulation of T cell proliferation
NCBI Summary:	This gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
UniProt Code:	P15813
NCBI GenInfo Identifier:	115964
NCBI Gene ID:	912
NCBI Accession:	P15813.1
UniProt Related Accession:	P15813
Molecular Weight:	~ 37kDa
NCBI Full Name:	Antigen-presenting glycoprotein CD1d
NCBI Synonym Full Names:	CD1d molecule
NCBI Official Symbol:	CD1D
NCBI Official Synonym Symbols:	R3; CD1A; R3G1
NCBI Protein Information:	antigen-presenting glycoprotein CD1d
UniProt Protein Name:	Antigen-presenting glycoprotein CD1d
UniProt Synonym Protein Names:	R3G1; CD_antigen: CD1d
Protein Family:	Antigen-presenting glycoprotein
UniProt Gene Name:	CD1D
UniProt Entry Name:	CD1D_HUMAN

	Western blot analysis of extracts of various cell lines, using CD1D antibody (CAB1760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of HeLa cells using CD1D antibody (CAB1760). Blue: DAPI for nuclear staining.

Antibodies

Anti-CD1D Antibody (CAB13475)

CD1D Antibody (PACO06868)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)