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Western blot analysis of various lysates, using CD226/DNAM-1 Rabbit mAb (CAB23200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

CD226/DNAM-1 Monoclonal Antibody (CAB23200)

Datasheet MSDS
SKU CAB23200
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human
Host Species Rabbit
Synonyms PTA1, DNAM1, DNAM-1, TLiSA1, CD226/DNAM-1
€131 - €457

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Description

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CD226/DNAM-1 Monoclonal Antibody (CAB23200)

The CD226/DNAM-1 Monoclonal Antibody (CAB23200) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced through hybridoma technology, provides high specificity and sensitivity in detecting CD226 protein in various cell types and tissues.CD226, also known as DNAX accessory molecule-1, is a co-stimulatory molecule expressed on T cells, NK cells, and other immune cell types. Its interaction with its ligands, such as CD155 (PVR) and CD112 (nectin-2), plays a crucial role in enhancing immune responses and promoting cell-mediated cytotoxicity.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:CD226/DNAM-1 Monoclonal Antibody
SKU:CAB23200
Size:20μL, 100μL
Reactivity:Human
Clone Number:ARC60137
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:Email for sequence
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PTA1, DNAM1, DNAM-1, TLiSA1, CD226/DNAM-1
Positive Sample:HEL
Cellular Localization:Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:39kDa
Observed MW:70-100kDa

This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID10666
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using CD226/DNAM-1 Rabbit mAb (CAB23200) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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