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Western blot analysis of various lysates, using CD23 antibody (CAB23092) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

CD23 Monoclonal Antibody (CAB23092)

Datasheet MSDS
SKU CAB23092
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human
Host Species Rabbit
Synonyms CD23, FCE2, CD23A, IGEBF, CLEC4J, FCErII, BLAST-2
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

CD23 Monoclonal Antibody (CAB23092)

The CD23 Monoclonal Antibody (CAB23092) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, created using hybridoma technology, specifically targets the CD23 protein in human samples and is validated for use in various applications, including immunohistochemistry and flow cytometry.CD23, also known as Fc epsilon RII, plays a critical role in the regulation of IgE production and the activation of B-cells.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:CD23 Monoclonal Antibody
SKU:CAB23092
Size:20μL, 100μL
Reactivity:Human
Clone Number:ARC55626
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:Email for sequence
Tested Applications:WB ELISA
Recommended Dilution:
WB1:1000 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CD23, FCE2, CD23A, IGEBF, CLEC4J, FCErII, BLAST-2
Positive Sample:Raji
Cellular Localization:Cell Membrane, Lipid-Anchor, Secreted, Single-Pass Type Ii Membrane Protein.
Calculated MW:36kDa
Observed MW:47kDa

The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Purification MethodAffinity purification
Gene ID2208
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using CD23 Rabbit mAb (CAB23092) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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