Anti-CD247 Antibody (CAB2058)

Antibody Name:	Anti-CD247 Antibody
Antibody SKU:	CAB2058
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-164 of human CD247 (NP_932170.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-164 of human CD247 (NP_932170.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FLRV KFSR SADA PAYQ QGQN QLYN ELNL GRRE EYDV LDKR RGRD PEMG GKPQ RRKN PQEG LYNE LQKD KMAE AYSE IGMK GERR RGKG HDGL YQGL STAT KDTY DALH MQAL PPR
Gene ID:	919
Uniprot:	P20963
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	18kDa
Observed MW:	17kDa

Synonyms:

CD247, CD3-ZETA, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCRZ

Background:

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

UniProt Protein Function:	CD3Z: a T cell surface glycoprotein that is a component of the T cell antigen receptor. Plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the CD3-epsilon results in impaired immune response. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Receptor, misc.; Membrane protein, integral Chromosomal Location of Human Ortholog: 1q24.2 Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; integral to membrane; alpha-beta T cell receptor complex Molecular Function:identical protein binding; protein binding; protein homodimerization activity; transmembrane receptor activity Biological Process: regulation of immune response; viral reproduction; T cell costimulation; innate immune response; T cell receptor signaling pathway; regulation of defense response to virus by virus Disease: Immunodeficiency 25
NCBI Summary:	The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P20963
NCBI GenInfo Identifier:	4557431
NCBI Gene ID:	919
NCBI Accession:	NP_000725.1
UniProt Secondary Accession:	P20963,Q5VX13, Q8TAX4, B1AK49,
UniProt Related Accession:	P20963,A46461
Molecular Weight:	18,696 Da
NCBI Full Name:	T-cell surface glycoprotein CD3 zeta chain isoform 2
NCBI Synonym Full Names:	CD247 molecule
NCBI Official Symbol:	CD247
NCBI Official Synonym Symbols:	T3Z; CD3H; CD3Q; CD3Z; TCRZ; CD3-ZETA
NCBI Protein Information:	T-cell surface glycoprotein CD3 zeta chain; CD3zeta chain; TCR zeta chain; CD247 antigen, zeta subunit; T-cell receptor T3 zeta chain; CD3Z antigen, zeta polypeptide (TiT3 complex); T-cell antigen receptor complex, zeta subunit of CD3
UniProt Protein Name:	T-cell surface glycoprotein CD3 zeta chain
UniProt Synonym Protein Names:	T-cell receptor T3 zeta chain
UniProt Gene Name:	CD247
UniProt Entry Name:	CD3Z_HUMAN

	Western blot analysis of extracts of mouse skeletal muscle, using CD247 antibody (CAB2058) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded Human esophageal cancer using CD247 antibody (CAB2058) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human tonsil using CD247 antibody (CAB2058) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using CD247 antibody (CAB2058). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-CD3 zeta Antibody (CAB11157)	CD3 zeta Colorimetric Cell-Based ELISA Kit
Phospho-CD247 (Y142) Antibody (PACO05713)	CD3 zeta (Phospho-Tyr142) Colorimetric Cell-Based ELISA Kit
CD247 Antibody (PACO05714)
CD247 Antibody (PACO07413)
CD247 Antibody (PACO08276)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)