Anti-CD27 Antibody (CAB1945)
- SKU:
- CAB1945
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-CD27 Antibody |
| Antibody SKU: | CAB1945 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-191 of human CD27 (NP_001233.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Raji, HeLa, Jurkat, HL-60, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-191 of human CD27 (NP_001233.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ATPA PKSC PERH YWAQ GKLC CQMC EPGT FLVK DCDQ HRKA AQCD PCIP GVSF SPDH HTRP HCES CRHC NSGL LVRN CTIT ANAE CACR NGWQ CRDK ECTE CDPL PNPS LTAR SSQA LSPH PQPT HLPY VSEM LEAR TAGH MQTL ADFR QLPA RTLS THWP PQRS LCSS DFIR |
| Gene ID: | 939 |
| Uniprot: | P26842 |
| Cellular Location: | Membrane, Single-pass type I membrane protein |
| Calculated MW: | 29kDa |
| Observed MW: | 50-55kDa |
| Synonyms: | CD27, S152, S152. LPFS2, T14, TNFRSF7, Tp55, S152.LPFS2 |
| Background: | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. |
| UniProt Protein Function: | CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Apoptosis; Receptor, cytokine Chromosomal Location of Human Ortholog: 12p13 Cellular Component: integral to plasma membrane; neuron projection; nucleus; plasma membrane Molecular Function:caspase inhibitor activity; protein binding; tumor necrosis factor receptor activity Biological Process: brain development; immune response; induction of apoptosis via death domain receptors; inflammatory response; negative regulation of apoptosis; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; regulation of cell proliferation; response to lipopolysaccharide; tumor necrosis factor-mediated signaling pathway Disease: Lymphoproliferative Syndrome 2 |
| NCBI Summary: | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P26842 |
| NCBI GenInfo Identifier: | 269849546 |
| NCBI Gene ID: | 939 |
| NCBI Accession: | P26842.2 |
| UniProt Secondary Accession: | P26842,B2RDZ0, |
| UniProt Related Accession: | P26842 |
| Molecular Weight: | 29,137 Da |
| NCBI Full Name: | CD27 antigen |
| NCBI Synonym Full Names: | CD27 molecule |
| NCBI Official Symbol: | CD27Â Â |
| NCBI Official Synonym Symbols: | T14; S152; Tp55; TNFRSF7; S152. LPFS2Â Â |
| NCBI Protein Information: | CD27 antigen |
| UniProt Protein Name: | CD27 antigen |
| UniProt Synonym Protein Names: | CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7; CD_antigen: CD27 |
| Protein Family: | CD27 antigen |
| UniProt Gene Name: | CD27Â Â |
| UniProt Entry Name: | CD27_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CD27 antibody (CAB1945) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat spleen using CD27 antibody (CAB1945) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using CD27 antibody (CAB1945) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of Jurkat cells using CD27 antibody (CAB1945) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of rat thymus using CD27 antibody (CAB1945) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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