The CD40L Antibody (CAB0327) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. It binds specifically to the CD40 Ligand protein, allowing for accurate detection and analysis in various cell types.CD40 Ligand, also known as CD154, is a key molecule involved in the regulation of immune responses, particularly in the activation of B cells and antigen-presenting cells. Dysregulation of the CD40 Ligand pathway has been implicated in various immune-related disorders, including autoimmune diseases and certain types of cancer.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CD40L Antibody
SKU:
CAB0327
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cell Membrane, Secreted, Single-Pass Type Ii Membrane Protein.
Calculated MW:
29kDa
Observed MW:
25-30kDa/17kDa
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Purification Method
Affinity purification
Gene ID
959
RRID
AB_2757128
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CD40L Rabbit pAb (CAB0327) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
