Anti-CD44 Antibody (CAB0340)

Antibody Name:	Anti-CD44 Antibody
Antibody SKU:	CAB0340
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 346-385 of human CD44 (NP_000601.3).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	K-562, HeLa, HT-1080

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 346-385 of human CD44 (NP_000601.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DVDR NGTT AYEG NWNP EAHP PLIH HEHH EEEE TPHS TSTI
Gene ID:	960
Uniprot:	P16070
Cellular Location:	Cell membrane, Single-pass type I membrane protein
Calculated MW:	3kDa/15kDa/32-53kDa/73-81kDa
Observed MW:	82kDa

Synonyms:

CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44, CD44

Background:

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

UniProt Protein Function:	CD44: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Interacts with PKN2. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. 19 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 11p13 Cellular Component: Golgi apparatus; focal adhesion; cell surface; basolateral plasma membrane; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane Molecular Function:collagen binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; hyaluronic acid binding; hyalurononglucosaminidase activity Biological Process: extracellular matrix organization and biogenesis; Wnt receptor signaling pathway; positive regulation of heterotypic cell-cell adhesion; glycosaminoglycan metabolic process; cytokine and chemokine mediated signaling pathway; cell-matrix adhesion; pathogenesis; negative regulation of caspase activity; positive regulation of peptidyl-serine phosphorylation; hyaluronan catabolic process; extracellular matrix disassembly; negative regulation of DNA damage response, signal transduction by p53 class mediator; cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; ureteric bud branching; cartilage development; carbohydrate metabolic process; blood coagulation; leukocyte migration; healing during inflammatory response; hyaluronan metabolic process; negative regulation of apoptosis Disease: Blood Group, Indian System
NCBI Summary:	The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]
UniProt Code:	P16070
NCBI GenInfo Identifier:	308153615
NCBI Gene ID:	960
NCBI Accession:	P16070.3
UniProt Secondary Accession:	P16070,O95370, P22511, Q04858, Q13419, Q13957, Q13958 Q13959, A5YRN9, B6EAT9, D3DR12, D3DR13,
UniProt Related Accession:	P16070
Molecular Weight:	81,538 Da
NCBI Full Name:	CD44 antigen
NCBI Synonym Full Names:	CD44 molecule (Indian blood group)
NCBI Official Symbol:	CD44
NCBI Official Synonym Symbols:	IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
NCBI Protein Information:	CD44 antigen; epican; Hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; heparan sulfate proteoglycan; cell surface glycoprotein CD44; extracellular matrix receptor III; chondroitin sulfate proteoglycan 8; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; homing function and Indian blood group system
UniProt Protein Name:	CD44 antigen
UniProt Synonym Protein Names:	CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I
Protein Family:	CD44 antigen
UniProt Gene Name:	CD44
UniProt Entry Name:	CD44_HUMAN

	Western blot analysis of extracts of various cell lines, using CD44 antibody (CAB0340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human tonsil using CD44 antibody (CAB0340) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using CD44 antibody (CAB0340) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using CD44 antibody (CAB0340) at dilution of 1:200 (40x lens).
	Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug CD44 antibody (CAB0340). Western blot was performed from the immunoprecipitate using CD44 antibody (CAB0340) at a dilition of 1:1000.

Antibodies	ELISA Kits
Human CD44 Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated) [P2A1](AGEL0875)	Human CD44 ELISA Kit
Human CD44 Monoclonal Antibody (AF647 Conjugated) [P2A1](AGEL0876)	Human CD44 PharmaGenie ELISA Kit
AF/LE Purified Human CD44 Monoclonal Antibody [J110](AGEL2101)	Human CD44 antigen (CD44) ELISA Kit
Human CD44 Monoclonal Antibody (PE Conjugated) [J110](AGEL2102)
Human CD44 Monoclonal Antibody (APC Conjugated) [J110](AGEL2103)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)