The Lysozyme (LYZ) Antibody (CAB13511) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to lysozyme and is validated for use in various applications such as immunohistochemistry and ELISA.Lysozyme is known for its ability to break down bacterial cell walls, making it a key player in the innate immune system. By targeting lysozyme, researchers can explore its role in host defense, inflammation, and infection.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Lysozyme (LYZ) Antibody
SKU:
CAB13511
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LZM, LYZF1, Lysozyme (LYZ)
Positive Sample:
HL-60, U-937, Mouse lung
Cellular Localization:
Secreted.
Calculated MW:
17kDa
Observed MW:
13kDa
This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis.
Purification Method
Affinity purification
Gene ID
4069
RRID
AB_2760373
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Lysozyme (LYZ) Rabbit pAb (CAB13511) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of various lysates using Lysozyme (LYZ) Rabbit pAb (CAB13511) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Mouse intestin using Lysozyme (LYZ) Rabbit pAb (CAB13511) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence analysis of RAW264.7 cells using Lysozyme (LYZ) Rabbit mAb (CAB13511) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
