The Lysozyme (LYZ) Monoclonal Antibody (CAB10972) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced through hybridoma technology, specifically targets lysozyme and is highly sensitive in detecting its presence in various samples.Lysozyme is found in a variety of biological fluids and tissues, making it a valuable marker for studying immune responses, bacterial infections, and other pathological conditions. The CAB10972 antibody can be used in a range of applications, including Western blotting, immunohistochemistry, and ELISA, allowing for precise and reliable detection of lysozyme expression.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Lysozyme (LYZ) Monoclonal Antibody
SKU:
CAB10972
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC57153
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LZM, LYZF1, Lysozyme (LYZ)
Positive Sample:
U-937, RAW 264.7, Mouse lung, Rat lung
Cellular Localization:
Secreted.
Calculated MW:
17kDa
Observed MW:
15kDa
This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk,and is also present in spleen,lung,kidney,white blood cells,plasma,saliva,and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis.
Purification Method
Affinity purification
Gene ID
4069
RRID
AB_2758350
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Lysozyme (LYZ) Rabbit mAb (CAB10972)at 1:60000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): HeLa Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Lysozyme (LYZ) Rabbit mAb (CAB10972) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Lysozyme (LYZ) Rabbit mAb (CAB10972) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Lysozyme (LYZ) Rabbit mAb (CAB10972) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
