Anti-CD68 Antibody (CAB15037)

Antibody Name:	Anti-CD68 Antibody
Antibody SKU:	CAB15037
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 16-200 of human CD68 (NP_001242.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	U-937, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 16-200 of human CD68 (NP_001242.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AAQG TGND CPHK KSAT LLPS FTVT PTVT ESTG TTSH RTTK SHKT TTHR TTTT GTTS HGPT TATH NPTT TSHG NVTV HPTS NSTA TSQG PSTA THSP ATTS HGNA TVHP TSNS TATS PGFT SSAH PEPP PPSP SPSP TSKE TIGD YTWT NGSQ PCVH LQAQ IQIR VMYT TQGG GEAW GISV LNPN K
Gene ID:	968
Uniprot:	P34810
Cellular Location:	Cell membrane, Endosome membrane, Lysosome membrane, Single-pass type I membrane protein, Single-pass type I membrane protein
Calculated MW:	31kDa/34kDa/37kDa
Observed MW:	70-80KDa

Synonyms:

CD68, GP110, LAMP4, SCARD1

Background:

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms.

UniProt Protein Function:	CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell surface; Membrane protein, integral Chromosomal Location of Human Ortholog: 17p13 Cellular Component: membrane; lysosomal membrane; plasma membrane; integral to membrane; endosome membrane
NCBI Summary:	This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	P34810
NCBI GenInfo Identifier:	147744552
NCBI Gene ID:	968
NCBI Accession:	P34810.2
UniProt Secondary Accession:	P34810,Q53HR6, Q53XI3, Q96BI7, B4DVT4,
UniProt Related Accession:	P34810
Molecular Weight:	354
NCBI Full Name:	Macrosialin
NCBI Synonym Full Names:	CD68 molecule
NCBI Official Symbol:	CD68
NCBI Official Synonym Symbols:	GP110; LAMP4; SCARD1
NCBI Protein Information:	macrosialin; CD68 antigen; macrophage antigen CD68; scavenger receptor class D, member 1
UniProt Protein Name:	Macrosialin
UniProt Synonym Protein Names:	Gp110; CD_antigen: CD68
Protein Family:	Macrosialin
UniProt Gene Name:	CD68
UniProt Entry Name:	CD68_HUMAN

	Western blot analysis of extracts of U-937 cells, using CD68 Rabbit pAb (CAB15037) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Western blot analysis of extracts of Mouse lung, using CD68 Rabbit pAb (CAB15037) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human liver using CD68 antibody (CAB15037) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of RAW264.7 cells using CD68 antibody (CAB15037) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-CD68 Antibody (CAB13286)	Human CD68 / Macrosialin ELISA Kit
Anti-CD68 Antibody (CAB5515)	Human Macrosialin (CD68) ELISA Kit
Anti-CD68 Antibody (CAB6554)
CD68 Antibody (PACO01923)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)