Anti-CDC34 Antibody (CAB5457)

Antibody Name:	Anti-CDC34 Antibody
Antibody SKU:	CAB5457
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-236 of human CDC34 (NP_004350.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, C6, SH-SY5Y, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-236 of human CDC34 (NP_004350.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MARP LVPS SQKA LLLE LKGL QEEP VEGF RVTL VDEG DLYN WEVA IFGP PNTY YEGG YFKA RLKF PIDY PYSP PAFR FLTK MWHP NIYE TGDV CISI LHPP VDDP QSGE LPSE RWNP TQNV RTIL LSVI SLLN EPNT FSPA NVDA SVMY RKWK ESKG KDRE YTDI IRKQ VLGT KVDA ERDG VKVP TTLA EYCV KTKA PAPD EGSD LFYD DYYE DGEV EEEA DSCF GDDE DDSG TEES
Gene ID:	997
Uniprot:	P49427
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	26kDa
Observed MW:	32KDa

Synonyms:	CDC34, E2-CDC34, UBC3, UBCH3, UBE2R1
Background:	The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.

UniProt Protein Function:	CDC34: a member of the ubiquitin-conjugating enzyme family. Is a part of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which is required for ubiquitin-mediated degradation of cell cycle G1 regulators. Required for the initiation of DNA replication.
UniProt Protein Details:	Protein type:Ligase; Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:ATP binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity Biological Process: DNA replication initiation; G1/S transition of mitotic cell cycle; positive regulation of neuron apoptosis; proteasomal ubiquitin-dependent protein catabolic process; protein modification process; protein polyubiquitination; protein ubiquitination
NCBI Summary:	The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008]
UniProt Code:	P49427
NCBI GenInfo Identifier:	2507505
NCBI Gene ID:	997
NCBI Accession:	P49427.2
UniProt Secondary Accession:	P49427,A8K689,
UniProt Related Accession:	P49427
Molecular Weight:	26,737 Da
NCBI Full Name:	Ubiquitin-conjugating enzyme E2 R1
NCBI Synonym Full Names:	cell division cycle 34
NCBI Official Symbol:	CDC34
NCBI Official Synonym Symbols:	UBC3; UBCH3; UBE2R1; E2-CDC34
NCBI Protein Information:	ubiquitin-conjugating enzyme E2 R1
UniProt Protein Name:	Ubiquitin-conjugating enzyme E2 R1
UniProt Synonym Protein Names:	(E3-independent) E2 ubiquitin-conjugating enzyme R1 (EC:2.3.2.24
Protein Family:	Ubiquitin-conjugating enzyme
UniProt Gene Name:	CDC34
UniProt Entry Name:	UB2R1_HUMAN

	Western blot analysis of extracts of Mouse testis, using CDC34 antibody (CAB5457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts of various cell lines, using CDC34 antibody (CAB5457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of HeLa cells using CDC34 antibody (CAB5457).

Antibodies

CDC34 Antibody (PACO00569)

CDC34 Antibody (PACO01902)

CDC34 Antibody (PACO08315)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)