The CDK9 Antibody (CAB1564) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been validated for its specificity and sensitivity in Western blot applications, specifically targeting the CDK9 protein.CDK9 is a crucial kinase that forms the core of the positive transcription elongation factor b (P-TEFb) complex, which promotes RNA polymerase II-mediated transcription elongation. Dysregulation of CDK9 activity has been implicated in various diseases, including cancer and HIV infection, making it an important target for therapeutic intervention.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CDK9 Antibody
SKU:
CAB1564
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TAK, C-2k, CTK1, CDC2L4, PITALRE, CDK9
Positive Sample:
HeLa, 293T, MCF7, NIH/3T3, C6
Cellular Localization:
Cytoplasm, Nucleus, Pml Body.
Calculated MW:
43kDa
Observed MW:
40kDa/55kDa
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
Purification Method
Affinity purification
Gene ID
1025
RRID
AB_2732887
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CDK9 Rabbit pAb (CAB1564) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from C6 cells, using CDK9 Rabbit pAb (CAB1564) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
