Description
system_update_altDatasheet
| Product Name: | Cell Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit |
| Product Code: | MAES0464 |
| Product Size: | 48 Tests/96 Tests |
| Sample Type: | Cell samples (adherent or suspension) |
| Detection Wavelength: | 340 nm |
| Detection Principle: | Assay Kit |
| RUO: | Research-Use-Only |
| Sensitivity: | 2.85 U/L |
| Detection Range: | 2.85-119.04 U/L |
| Intra-assay CV: | 3.0-4.0% |
| Inter-assay CV: | 5.0-8.0% |
| Recovery Rate: | 99% |
Mitochondrial Complex I (NADH-CoQ Reductase) catalyzes the reaction of NADH with the ubiquinone substrate to generate NAD+ and reduced ubiquinone. This enzyme plays a crucial role in cellular energy metabolism and the electron transport chain. The activity of mitochondrial Complex I can be quantified by measuring the change in optical density at 340 nm, reflecting NADH consumption during the enzymatic reaction.
This kit employs a detection principle whereby mitochondrial Complex I catalyzes the oxidation of NADH to NAD+. The activity of mitochondrial Complex I is determined by measuring the change in optical density (ΔOD) at 340 nm over a 5-minute incubation period at 37 °C, which reflects the consumption of NADH substrate by the enzyme.
| Shelf Life: | 12 Months |
| Storage: | -20 °C |
| Assay Time: | 10 minutes (5 min sample incubation + 5 min reaction) |
| Research Area: | Enzymes, Cellular Metabolism |
