Description
CellQuant-Lux 3D Luciferase Assay System (ASRV00012)
CellQuant-Lux 3D is a single-reagent, homogeneous assay specifically formulated to accurately determine the number of viable cells in complex 3D cell culture models (microtissues and spheroids). It quantifies the ATP present, which serves as a marker for metabolically active cells. This ready-to-use reagent is based on the highly sensitive luciferase system and is formulated with an optimized reaction composition and significantly stronger lytic capacity to effectively penetrate and lyse dense microtissue cell masses. The assay is compatible with multiwell-plate formats and the homogeneous "add-mix-measure" procedure makes it ideal for automated High-Throughput Screening (HTS) using cell proliferation and cytotoxicity assays. Cell washing, removal of medium, and multiple pipetting steps are not required.
| Product Name: | CellQuant-Lux 3D Luciferase Assay System |
| SKU: | ASRV00012 |
| Detection Principle: | Luminescence |
| Step | Protocol |
| 1 | Prepare multiwell plates containing your microtissues in culture medium (we recommend the use of opaque wall microplates compatible with your luminometer). Optimize microtissue size, number as well as cell culture conditions prior to experimentation. |
| 2 | Add the test compound(s) to your experimental wells and incubate according to your established cell culture protocol. |
| 3 | Optional Background Control: Prepare control wells containing only culture medium (without cells) to determine the background luminescence signal. |
| 4 | Remove the cell culture plate to be tested from the incubator and equilibrate at room temperature for 30 min. |
| 5 | Add equal amount of CellQuant-Lux 3D which is equal to the volume of the cell culture to be tested. For example, when using a 96-well culture plate, add 100 μl CellQuant-Lux 3D to 100 μl cell culture to be tested. |
| 6 | Vigorously mix on a plate shaker (orbital) for 5 minutes to lyse the cells. |
| 7 | Incubate at room temperature for 10 min to stabilize the luminescence signal. |
| 8 | Detect luminescence. |
| Step | Protocol |
| Thawing | Incubate the CellQuant-Lux 3D at 2 to 8℃ or room temperature to thaw. The product can also be incubated at 22℃ in a water bath but the temperature should not exceed 25℃. |
| Equilibrate to room temperature | If the product is not thawed at room temperature, it should be placed in a 22℃ water bath to equilibrate to room temperature. Generally, it takes about 10 min for 10 ml pack size, 30 min for 100 ml pack size or 100 min for 400 ml pack size. |
| Mix | Gently invert 5 times before use, to mix the solution evenly. |
| Product | Code | Pack Size | Assays Per Pack |
| CellQuant-Lux 3D | ASRV00012-10 | 10ml | 100 assays/96-well plate 400 assays/384-well plate |
| CellQuant-Lux 3D | ASRV00012-100 | 100ml | 1000 assays/96-well plate 4000 assays/384-well plate |
| CellQuant-Lux 3D | ASRV00012-400 | 400ml | 4000 assays/96-well plate 16,000 assays/384-well plate |
