The CHAT Antibody (CAB2495) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbit immunoglobulins, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the CD300A protein, this antibody enables detection and analysis in various cell types, making it a valuable tool for studies in immunology and cancer research.CD300A, also referred to as an immune inhibitory receptor, plays a crucial role in immune homeostasis by regulating inflammation and suppressing allergic reactions.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CHAT Antibody
SKU:
CAB2495
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.
Purification Method
Affinity purification
Gene ID
1103
RRID
AB_2764386
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CHAT Rabbit pAb (CAB2495). Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using CHAT Rabbit pAb (CAB2495) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
