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Western blot analysis of extracts from K-562 cells, using CHD1 Rabbit mAb (CAB23240) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

CHD1 Monoclonal Antibody (CAB23240)

Datasheet MSDS
SKU CAB23240
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human
Host Species Rabbit
Synonyms CHD-1, PILBOS, CHD1
$173 - $603

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Description

system_update_altDatasheetsystem_update_altMSDS

CHD1 Monoclonal Antibody (CAB23240)

The CHD1 Monoclonal Antibody (CAB23240) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, has high specificity for CHD1 and has been validated for use in various applications such as immunofluorescence and immunohistochemistry.CHD1 is essential for maintaining genome stability and proper gene expression, making it a key player in cellular processes such as cell proliferation and differentiation. Dysregulation of CHD1 has been linked to various diseases, including cancer and developmental disorders.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:CHD1 Monoclonal Antibody
SKU:CAB23240
Size:20μL, 100μL
Reactivity:Human
Clone Number:ARC59018
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:WB ELISA
Recommended Dilution:
WB1:1000 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CHD-1, PILBOS, CHD1
Positive Sample:K-562
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:197kDa
Observed MW:250kDa

The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

Purification MethodAffinity purification
Gene ID1105
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from K-562 cells using CHD1 Rabbit mAb (CAB23240) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
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