Western blot analysis of extracts from HeLa cells, using Phospho-Chk1-S345 Rabbit mAb (CAB21009) at1:20000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-Chk1-S345 Monoclonal Antibody (CAB21009) is a high-quality antibody developed for reliable detection and analysis of target proteins. CHK1 is a key regulator of the cell cycle and is activated in response to DNA damage, serving to halt cell cycle progression and allow for DNA repair. Phosphorylation of CHK1 at serine 345 is a critical event in its activation and function.This monoclonal antibody, developed and validated for use in various applications including Western blot, immunofluorescence, and immunoprecipitation, specifically recognizes CHK1 when phosphorylated at serine 345.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-Chk1-S345 Monoclonal Antibody
SKU:
CAB21009
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC56803
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
SFSQ P
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
1111
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells,using Phospho-Chk1-S345 Rabbit mAb (CAB21009) at1:2000 dilution. HeLa cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from NIH/3T3 cells,using Phospho-Chk1-S345 Rabbit mAb (CAB21009) at1:2000 dilution. NIH/3T3 cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
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